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High discrimination between genotypes with 100% sensitivity for FV:Q506. Reduces need for PCR determination.
Applicable to anticoagulant treated patients.

COATEST® APCTM RESISTANCE V


An APTT-based kit for screening of factor V-related APC resistance. The high sensitivity and specificity of the test for the factor V:Q506 mutation is obtained by prediluting the sample plasma with an excess of V-DEF Plasma.
The test design makes it possible to discriminate between heterozygous and homozygous factor V genotypes.
It also allows for analysis of plasma from patients on heparin or oral anticoagulant therapy.

Measurement Principle

One volume of plasma is prediluted with four volumes of V-DEF Plasma. The dilution is then incubated with the APTT reagent for a standard period of time. Coagulation is triggered by the addition of CaCl2 in the absence and presence of exogenous APC and the time for clot formation is recorded.



Reagents and their stability when opened

APC/CaCl2
4x2 ml
5 days
2-8°C
8 hours
15-25°C
APTT reagent
1x16 ml
1 week
15-25°C
1 month
2-8°C
CaCl2
1x8 ml
1 month
2-8°C
1 week
15-25°C
V-DEF Plasma
4x4 ml
8 hours
15-25°C
24 hours
2-8°C
3 months
-20°C
APC/CaCl2
4x2 ml
3 months
-20°C
Control Plasma Level 1
1x1 ml
6 hours
2-25°C
3 months
-20°C
Control Plasma Level 2
1x1 ml
6 hours
2-25°C
3 months
-20°C

Determinations

80-160

Part Number: 82 3120 63

COATEST® APCTM RESISTANCE V-S


An APTT-based kit for screening of factor V-related APC resistance. The high sensitivity and specificity of the test for the factor V:Q506 mutation is obtained by prediluting the sample plasma with an excess of V-DEF Plasma.
The test design makes it possible to discriminate between heterozygous and homozygous factor V genotypes.
It also allows for analysis of plasma from patients on heparin or oral anticoagulant therapy.

Measurement Principle

One volume of plasma is prediluted with four volumes of V-DEF Plasma. The dilution is then incubated with the APTT reagent for a standard period of time. Coagulation is triggered by the addition of CaCl2 in the absence and presence of exogenous APC and the time for clot formation is recorded.



Reagents and their stability when opened

V-DEF Plasma
2x4 ml
8 hours
15-25°C
24 hours
2-8°C
3 months
-20°C
CaCl2
2x2 ml
1 week
15-25°C
1 month
2-8°C
APTT reagent
2x4 ml
1 week
15-25°C
1 month
2-8°C
APC/CaCl2
2x2 ml
8 hours
15-25°C
5 days
2-8°C
3 months
-20°C
Control Plasma Level 1
1x1 ml
6 hours
2-25°C
3 months
-20°C
Control Plasma Level 2
1x1 ml
6 hours
2-25°C
3 months
-20°C

Determinations

40-80

Part Number: 82 3138 63